FNC Coating Mix is a specially formulated serum-free tissue culture reagent containing fibronectin, collagen, and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostate and breast epithelial cells are being propagated.
Mammalian cell cultures will ach and grow more effectively on the FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. Athena offers several different serum-free media that when used in conjunction with FNC Coating Mix increase the growth of cell cultures dramatically.
FNC Coating Mix is a specially formulated serum-free tissue culture reagent containing fibronectin, collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated.
human FNC Coating Mix
Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaES offers several different serum-free media that when used in conjunction with FNC Coating Mix, increase the growth of cell cultures dramatically.
Order #
Product Name
Type
Unit
Price
0407
FNC Coating Mix
Liquid (ready-to-use)
50mL
$91.00
Inquire about availability of 50mL size.
0407
FNC Coating Mix
Liquid (ready-to-use)
100mL
$182.00
Description
FNC Coating Mix is a specially formulated reagent that dramatically increases the attachment of cells to the plastic substratum used for culturing mammalian cell lines. Increased cell attachment facilitates and encourages growth in adherent cell cultures. Many fastidious cell types such as human prostate and breast epithelial cells will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium.
The photograph to left shows the difference in monolayer development with and without FNC Coating Mix®. BRFF-55T prostate cancer cells were grown in HPC1™ medium at the same seed concentration for 3 days at 37°C, 5% CO2. The flask shown on left was pre-coated with FNC Coating Mix™ whereas the flask on the right was not pre-treated. Through image analysis, there was approximately 85% greater monolayer development in the flask coated with FNC than in the non-treated flask.
1. To coat the growth surface, add 0.2 mL FNC Coating Mix per square centimeter of the surface area of the culture vessel (For Example: use 5 mL for a T-25 flask). The surface should be completely covered with a layer of liquid.
2. Incubate for 30 seconds at room temperature and remove the coating mix with a pipet. Residual FNC Coating Mix will not adversely affect the viability of the cells to be plated. Do not return any “used” coating mix to the original container of FNC. This will compromise the performance of the product. The coating can be done
immediately before seeding the cells or up to one hour beforehand
