human FNC Coating Mix

Human FNC Coating Mix fibronectin, collagen, and albumin

FNC Coating Mix is a specially formulated serum-free tissue culture reagent containing fibronectin, collagen, and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostate and breast epithelial cells are being propagated.
Mammalian cell cultures will ach and grow more effectively on the FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. Athena offers several different serum-free media that when used in conjunction with FNC Coating Mix increase the growth of cell cultures dramatically.
FNC Coating Mix is a specially formulated serum-free tissue culture reagent containing fibronectin, collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated.
human FNC Coating Mix
human FNC Coating Mix
Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaES offers several different serum-free media that when used in conjunction with FNC Coating Mix, increase the growth of cell cultures dramatically.
Order # Product Name Type Unit Price
0407 FNC Coating Mix Liquid (ready-to-use) 50mL $91.00
Inquire about availability of 50mL size.
0407 FNC Coating Mix Liquid (ready-to-use) 100mL $182.00

Description

FNC Coating Mix is a specially formulated reagent that dramatically increases the attachment of cells to the plastic substratum used for culturing mammalian cell lines. Increased cell attachment facilitates and encourages growth in adherent cell cultures. Many fastidious cell types such as human prostate and breast epithelial cells will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium.

The photograph to left  shows the difference in monolayer development with and without FNC Coating Mix®. BRFF-55T prostate cancer cells were grown in HPC1™ medium at the same seed concentration for 3 days at 37°C, 5% CO2. The flask shown on left was pre-coated with FNC Coating Mix™ whereas the flask on the right was not pre-treated. Through image analysis, there was approximately 85% greater monolayer development in the flask coated with FNC than in the non-treated flask.

1. To coat the growth surface, add 0.2 mL FNC Coating Mix per square centimeter of the surface area of the culture vessel (For Example: use 5 mL for a T-25 flask). The surface should be completely covered with a layer of liquid.

2. Incubate for 30 seconds at room temperature and remove the coating mix with a pipet. Residual FNC Coating Mix will not adversely affect the viability of the cells to be plated. Do not return any “used” coating mix to the original container of FNC. This will compromise the performance of the product. The coating can be done
immediately before seeding the cells or up to one hour beforehand

 

Coating Buffer

abx098970-1vial 1 vial
EUR 184.8

Coating Buffer

RM01756 20mL
EUR 280

Coating Buffer

DS0236-100ML 1 unit
EUR 2.8
Description: Coating Buffer

Coating Buffer

DS0236-500ML 1 unit
EUR 12.71
Description: Coating Buffer

Coating Buffer

abx098970-100l 100 µl
EUR 93.75

Coating Buffer

abx098970-1ml 1 ml Ask for price

Coating Buffer

abx098970-200l 200 µl Ask for price

coating ab of 044

044-1 100µg
EUR 198

coating ab of 041

041-1 100µg
EUR 165

coating ab of 043

043-1 100µg
EUR 165

Coating Stabilizer

CSB125 125 ml
EUR 24.43

Coating Stabilizer

CSB500 500 ml
EUR 47.58

Coating Stabilizer

CSB999 1000 ml
EUR 86.15

Peptide Coating Kit

GWB-3F7464 Reagent Ask for price

iPSC Coating Solution

cAP-50 100ml
EUR 178.2

Quick Coating Solution

cAP-01 240ml
EUR 133.65

Matrigel Coating Solution

cAP-58 100ml
EUR 178.2

Anti-CEA McAb (coating)

E61C00205a 1mg
EUR 287.5
Description: Available in various conjugation types.

Anti-CEA McAb (Coating)

E61C00207 1mg
EUR 287.5
Description: Available in various conjugation types.

Anti-AFP McAb (Coating)

E61C00305 1mg
EUR 1125
Description: Available in various conjugation types.

Anti-TPA McAb (coating)

E61C01301 1mg
EUR 1125
Description: Available in various conjugation types.

Anti-CK8 McAb (coating)

E61C01701 1mg
EUR 1125
Description: Available in various conjugation types.

Anti-PGI McAb (coating)

E61C01801 100ug
EUR 225
Description: Available in various conjugation types.

Anti-HE4 McAb (Coating)

E61C02601 100ug
EUR 225
Description: Available in various conjugation types.

Anti-HE4 McAb (Coating)

E61C02601a 1mg
EUR 1125
Description: Available in various conjugation types.

Anti-HBS McAb (coating)

E61H00202 1mg
EUR 115
Description: Available in various conjugation types.

Anti-HBe McAb (coating)

E61H00401 1mg
EUR 143.75
Description: Available in various conjugation types.

Anti-HBc McAb (coating)

E61H00601 1mg
EUR 143.75
Description: Available in various conjugation types.

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