human FNC Coating Mix

Human FNC Coating Mix fibronectin, collagen, and albumin

FNC Coating Mix is a specially formulated serum-free tissue culture reagent containing fibronectin, collagen, and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostate and breast epithelial cells are being propagated.
Mammalian cell cultures will ach and grow more effectively on the FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. Athena offers several different serum-free media that when used in conjunction with FNC Coating Mix increase the growth of cell cultures dramatically.
FNC Coating Mix is a specially formulated serum-free tissue culture reagent containing fibronectin, collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation, especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated.
human FNC Coating Mix
human FNC Coating Mix
Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaES offers several different serum-free media that when used in conjunction with FNC Coating Mix, increase the growth of cell cultures dramatically.
Order # Product Name Type Unit Price
0407 FNC Coating Mix Liquid (ready-to-use) 50mL $91.00
Inquire about availability of 50mL size.
0407 FNC Coating Mix Liquid (ready-to-use) 100mL $182.00

Description

FNC Coating Mix is a specially formulated reagent that dramatically increases the attachment of cells to the plastic substratum used for culturing mammalian cell lines. Increased cell attachment facilitates and encourages growth in adherent cell cultures. Many fastidious cell types such as human prostate and breast epithelial cells will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium.

The photograph to left  shows the difference in monolayer development with and without FNC Coating Mix®. BRFF-55T prostate cancer cells were grown in HPC1™ medium at the same seed concentration for 3 days at 37°C, 5% CO2. The flask shown on left was pre-coated with FNC Coating Mix™ whereas the flask on the right was not pre-treated. Through image analysis, there was approximately 85% greater monolayer development in the flask coated with FNC than in the non-treated flask.

1. To coat the growth surface, add 0.2 mL FNC Coating Mix per square centimeter of the surface area of the culture vessel (For Example: use 5 mL for a T-25 flask). The surface should be completely covered with a layer of liquid.

2. Incubate for 30 seconds at room temperature and remove the coating mix with a pipet. Residual FNC Coating Mix will not adversely affect the viability of the cells to be plated. Do not return any “used” coating mix to the original container of FNC. This will compromise the performance of the product. The coating can be done
immediately before seeding the cells or up to one hour beforehand

 

FNC Coating Mix® - Human Proteins, 50 ml

0407H 50 ml
EUR 138

Coating Stabilizer

CSB125 125 ml
EUR 80

Coating Stabilizer

CSB500 500 ml
EUR 109

Coating Stabilizer

CSB999 1000 ml
EUR 157

Coating Buffer

abx098970-1vial 1 vial
EUR 154
  • Shipped within 5-7 working days.

Universal Coating Solution

AC002 20 ml
EUR 297

Binding/Coating Buffer (10X)

85R-124 250 ml
EUR 216
Description: ELISA buffer for optimal coating and binding of antibodies and antigens

Plate Coating Buffer-CB1

KF17336 500 ml
EUR 244

Coating Buffer, 20X, 100ML

X108-100ML 100ML
EUR 153

Coating Buffer, 20X, 10ML

X108-10ML 10ML
EUR 94

Blocking Buffer and Coating Stabilizer

abx090811-1000ml 1000 ml
EUR 523
  • Shipped within 5-10 working days.

Recombinant HTLV gp21 Protein (Coating) [2B3A]

VAng-Lsx0306-inquire inquire Ask for price
Description: HTLV gp21 Antigen [2B3A], Recombinant antigen.

dNTP Mix

G010 250 ul 10 mM each
EUR 73

dNTP Mix

G128 500 ul 10 mM each
EUR 82

dNTP Mix

G129 1.0 ml 10 mM each
EUR 101

NTP Mix

G943 250 ul 25 mM each
EUR 78

NTP Mix

G944 500 ul 25 mM each
EUR 97

NTP Mix

G945 1.0 ml 25 mM each
EUR 130

dNTP Mix

K6011105-100 100 ul (10 mM each)
EUR 86
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

dNTP Mix

K6011105-1000 1000 ul (10 mM each)
EUR 201
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

dNTP Mix

K6011105-200 200 ul (10 mM each)
EUR 93
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

dNTP Mix

K6011105-400 400 ul (10 mM each)
EUR 116
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

PCR Mix

L5051100 2.5 ml
EUR 107
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Coating Antibody or Protein stabilizer solution (10X)

PRTS-100 100 ml
EUR 286

Carbonate-bicarbonate Coating buffer pH 9.6, 100 ml

09-8922-100 100 ml
EUR 108

Carbonate-bicarbonate Coating buffer pH 9.6, 100 ml

09-8922-24 100 ml
EUR 82

ELISA Plate Coating buffer concentrate pH 7.4 (10X)

80050 50 ml
EUR 164

Glucose Nutrient Mix

109 150 g
EUR 67

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