proteinase k recombinant protein

Fungus-Derived Proteinase K Purified Recombinant

Product Disclaimer

These products are supplied for manufacturing, research and laboratory use only. Researchers and laboratory personnel intending to use any of these products for medical investigation on humans are solely responsible for such use and for compliance with the pertinent regulations of the United States Food and Drug Administration (US-FDA) and other regulations. We do not assume liability for damages resulting from the handling, use and/or disposal of these products, from their use in violation of patent or other rights or reliance upon this information.

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Thermo Scientific Proteinase K is a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations. It degrades proteins even in the presence of detergents. Proteinase K cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Application

  • This PCR grade Proteinase K is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.
  • The enzyme promotes cell lysis by activating a bacterial autolytic factor.
  • Proteinase K is also used for the analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.
  • The enzyme is particularly well suited for isolating nucleic acids for amplification reactions.
  • Proteinase K can be used to remove cellular debris during the preparation of colony lifts, and to treat tissue sections to ensure efficient probe infiltration during in situ hybridization.

Fungus-Derived Proteinase K Purified Recombinant from Innovative Research is prepared at 20 mg/ml in 10 mM Tris-HCl, 1 mM calcium acetate, pH 7.5 containing 50% glycerol. This is a liquid with a concentration of 400 Units/ml.

This product is useful providing cleavage of peptide bonds,and is able to catalyze peptide amide hydrolysis. It is tested to be free of DNase and RNase. Concentrated and ready to use liquid.

More Details:

  • Source: Yeast (Recombinant Tritiachium album Proteinase K)
  • Storage Conditions: -20°C

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.


Highlights

• Ready-to-use solution
• Active in a wide range of reaction conditions

Applications

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines
• Determination of enzyme localization
• Improving cloning efficiency of PCR products

proteinase k recombinant protein
proteinase k recombinant protein
Note

• The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to 4 M urea
• Ca2+ protects Proteinase K against autolysis, increases the thermal stability, and has a regulatory function for the substrate binding site of Proteinase K
• Stable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0
• Optimum activity at 50 to55°C
• Rapid denaturation of enzyme occurs at temperatures above 65°C

Features and Benefits

Proteinase K cleaves proteins between amino acids X and Y (X--Y), when X = an aliphatic, aromatic, or hydrophobic amino acid, and Y = any amino acid. The enzyme is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases.

  • Choose an effective tool for template preparation. Inactivate DNases and RNases of most species.
  • Count on consistent quality and performance. Stringent quality testing ensures optimal stability and high-level lot-to-lot performance.
  • Prepare samples over a wide range of conditions. The robust enzyme is stable over a wide pH range and is ideal for diverse applications.
  • Benefit from a contamination-free enzyme. The enzyme is tested for the absence of RNases and DNases, and is virtually free of DNA. It is especially suited for the isolation of PCR templates.

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