ELISA Kit for Immunoglobulin A (IgA)

ELISA Kit for Immunoglobulin A (IgA)

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Specificity

This assay has high sensitivity and excellent specificity for detection of Immunoglobulin A (IgA).
No significant cross-reactivity or interference between Immunoglobulin A (IgA) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Immunoglobulin A (IgA) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin A (IgA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-94 88
EDTA plasma(n=5) 89-103 97
heparin plasma(n=5) 96-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin A (IgA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin A (IgA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin A (IgA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 85-92% 94-102% 96-103%
EDTA plasma(n=5) 94-102% 95-105% 89-97% 87-99%
heparin plasma(n=5) 88-96% 92-99% 85-93% 89-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
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ELISA kit for Human IgA (Immunoglobulin A)

E-EL-H1355 1 plate of 96 wells
EUR 377
  • Gentaur's IgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human IgA (Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse IgA (Immunoglobulin A)

E-EL-M0690 1 plate of 96 wells
EUR 534
  • Gentaur's IgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgA. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Mouse IgA (Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Monkey IgA (Immunoglobulin A)

E-EL-MK0397 1 plate of 96 wells
EUR 520
  • Gentaur's IgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey IgA. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Monkey IgA (Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Porcine IgA (Immunoglobulin A)

E-EL-P1273 1 plate of 96 wells
EUR 520
  • Gentaur's IgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IgA. Standards or samples are added to the micro ELISA plate wells and combined with th
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Porcine IgA (Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat IgA (Immunoglobulin A)

E-EL-R0516 1 plate of 96 wells
EUR 534
  • Gentaur's IgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgA. Standards or samples are added to the micro ELISA plate wells and combined with the sp
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Rat IgA (Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rabbit IgA (Immunoglobulin A)

E-EL-RB1208 1 plate of 96 wells
EUR 520
  • Gentaur's IgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IgA. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Rabbit IgA (Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human Immunoglobulin A,IgA

EK0229 96 tests
EUR 578
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Immunoglobulin A,IgA in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Mouse IgA (Immunoglobulin A)

ELK2595 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobu
  • Show more
Description: A sandwich ELISA kit for detection of Immunoglobulin A from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Rat IgA (Immunoglobulin A)

ELK2596 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobu
  • Show more
Description: A sandwich ELISA kit for detection of Immunoglobulin A from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human IgA (Immunoglobulin A)

ELK1255 1 plate of 96 wells
EUR 372
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobu
  • Show more
Description: A sandwich ELISA kit for detection of Immunoglobulin A from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Gallus IgA (Immunoglobulin A)

ELK7653 1 plate of 96 wells
EUR 526
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobu
  • Show more
Description: A sandwich ELISA kit for detection of Immunoglobulin A from Chicken in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Gallus IgA (Immunoglobulin A)

ELK5469 1 plate of 96 wells
EUR 526
  • A monoclonal antibody specific to Immunoglobulin A (IgA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin A (IgA) and unlabeled Immunoglobulin A (IgA) (Standards or samples) wi
  • Show more
Description: A competitive Inhibition ELISA kit for detection of Immunoglobulin A from Chicken in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Pig IgA (Immunoglobulin A)

ELK5779 1 plate of 96 wells
EUR 526
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobu
  • Show more
Description: A sandwich ELISA kit for detection of Immunoglobulin A from Pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Cattle IgA (Immunoglobulin A)

ELK5827 1 plate of 96 wells
EUR 526
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobu
  • Show more
Description: A sandwich ELISA kit for detection of Immunoglobulin A from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse Secretory immunoglobulin A

KTE70438-48T 48T
EUR 332
  • Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a crucial role in the immune function of mucous membranes. IgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Secretory immunoglobulin A in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Secretory immunoglobulin A

KTE70438-5platesof96wells 5 plates of 96 wells
EUR 2115
  • Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a crucial role in the immune function of mucous membranes. IgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Secretory immunoglobulin A in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Secretory immunoglobulin A

KTE70438-96T 96T
EUR 539
  • Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a crucial role in the immune function of mucous membranes. IgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Secretory immunoglobulin A in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Sheep Immunoglobulin A (IgA)  Kit

KTE110079-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Sheep Immunoglobulin A (IgA)  Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Sheep Immunoglobulin A (IgA)  Kit

KTE110079-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Sheep Immunoglobulin A (IgA)  Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Sheep Immunoglobulin A (IgA)  Kit

KTE110079-96T 96T
EUR 572
Description: Quantitative sandwich ELISA for measuring Sheep Immunoglobulin A (IgA)  Kit in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human MIgA (Membrane Immunoglobulin A)

E-EL-H1080 1 plate of 96 wells
EUR 534
  • Gentaur's MIgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIgA. Standards or samples are added to the micro ELISA plate wells and combined with th
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human MIgA (Membrane Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human sIgA (Secretory Immunoglobulin A)

E-EL-H1275 1 plate of 96 wells
EUR 534
  • Gentaur's sIgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sIgA. Standards or samples are added to the micro ELISA plate wells and combined with th
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human sIgA (Secretory Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse MIgA (Membrane Immunoglobulin A)

E-EL-M0789 1 plate of 96 wells
EUR 534
  • Gentaur's MIgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIgA. Standards or samples are added to the micro ELISA plate wells and combined with th
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Description: A sandwich ELISA kit for quantitative measurement of Mouse MIgA (Membrane Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse sIgA (Secretory Immunoglobulin A)

E-EL-M1040 1 plate of 96 wells
EUR 534
  • Gentaur's sIgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sIgA. Standards or samples are added to the micro ELISA plate wells and combined with th
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Mouse sIgA (Secretory Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Porcine sIgA (Secretory Immunoglobulin A)

E-EL-P1528 1 plate of 96 wells
EUR 584
  • Gentaur's sIgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine sIgA. Standards or samples are added to the micro ELISA plate wells and combined with
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Porcine sIgA (Secretory Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat sIgA (Secretory Immunoglobulin A)

E-EL-R0875 1 plate of 96 wells
EUR 534
  • Gentaur's sIgA ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Rat sIgA (Secretory Immunoglobulin A) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Gallus sIgA (Secretory Immunoglobulin A)

ELK4908 1 plate of 96 wells
EUR 526
  • A monoclonal antibody specific to Secretory Immunoglobulin A (sIgA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretory Immunoglobulin A (sIgA) and unlabeled Secretory Immunoglobulin A
  • Show more
Description: A competitive Inhibition ELISA kit for detection of Secretory Immunoglobulin A from Chicken in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Rat sIgA (Secretory Immunoglobulin A)

ELK1548 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Immunoglobulin A (sIgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to
  • Show more
Description: A sandwich ELISA kit for detection of Secretory Immunoglobulin A from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse sIgA (Secretory Immunoglobulin A)

ELK1549 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Immunoglobulin A (sIgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to
  • Show more
Description: A sandwich ELISA kit for detection of Secretory Immunoglobulin A from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Human sIgA (Secretory Immunoglobulin A)

ELK1844 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Immunoglobulin A (sIgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to
  • Show more
Description: A sandwich ELISA kit for detection of Secretory Immunoglobulin A from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Cattle sIgA (Secretory Immunoglobulin A)

ELK6101 1 plate of 96 wells
EUR 526
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Immunoglobulin A (sIgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to
  • Show more
Description: A sandwich ELISA kit for detection of Secretory Immunoglobulin A from Cattle in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Pig sIgA (Secretory Immunoglobulin A)

ELK7387 1 plate of 96 wells
EUR 526
  • A monoclonal antibody specific to Secretory Immunoglobulin A (sIgA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretory Immunoglobulin A (sIgA) and unlabeled Secretory Immunoglobulin A
  • Show more
Description: A competitive Inhibition ELISA kit for detection of Secretory Immunoglobulin A from Pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Rat Secretory immunoglobulin A (sIgA)

KTE100261-48T 48T
EUR 354
  • Secretory IgA (IgA) consists of two IgA monomers joined by the J-chain and furthermore a secretory component. It is secreted in plasma cells based in the lamina propia of mucosal membranes. Synthesis of IgA is independent from the synthesis of serum
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Secretory immunoglobulin A (sIgA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Secretory immunoglobulin A (sIgA)

KTE100261-5platesof96wells 5 plates of 96 wells
EUR 2252
  • Secretory IgA (IgA) consists of two IgA monomers joined by the J-chain and furthermore a secretory component. It is secreted in plasma cells based in the lamina propia of mucosal membranes. Synthesis of IgA is independent from the synthesis of serum
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Secretory immunoglobulin A (sIgA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Secretory immunoglobulin A (sIgA)

KTE100261-96T 96T
EUR 572
  • Secretory IgA (IgA) consists of two IgA monomers joined by the J-chain and furthermore a secretory component. It is secreted in plasma cells based in the lamina propia of mucosal membranes. Synthesis of IgA is independent from the synthesis of serum
  • Show more
Description: Quantitative sandwich ELISA for measuring Rat Secretory immunoglobulin A (sIgA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Secretory immunoglobulin A (sIgA)

KTE10116-48T 48T
EUR 354
Description: Quantitative sandwich ELISA for measuring Bovine Secretory immunoglobulin A (sIgA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Bovine Secretory immunoglobulin A (sIgA)

KTE10116-5platesof96wells 5 plates of 96 wells
EUR 2252
Description: Quantitative sandwich ELISA for measuring Bovine Secretory immunoglobulin A (sIgA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

 

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