Angiostatin K1-3

Catalog Number: PRO-284

Synonyms: Angiostatin, Angiostatin Kringles 1-3, Ang K1-3.

Introduction

Ang K1-3 is a proteolytic fragment of plasminogen that contains the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active in relation to kringles 1-4. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth.

Description

Recombinant human angiostatin produced in E. coli is a single non-glycosylated polypeptide chain containing 259 amino acids and having a molecular mass of approximately 30.0 kDa. Ang K1-3 is purified by proprietary chromatographic techniques.

Fountain

Escherichia coli.

Physical Appearance

Sterile filtered white lyophilized (lyophilized) powder.

Formulation

Lyophilized from a filtered 0.2 µm (1.0 mg / ml) concentrated solution in 20 mM NaAc, pH 5.5, 4% mannitol.

Solubility

We recommend briefly centrifuging the vial before opening it to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA at a concentration of 0.1-1.0 mg/ml. Stock solutions should be distributed into working aliquots and stored at <-20 ° C. Additional dilutions should be made in suitable buffered solutions.

Stability

Lyophilized angiostatin K1-3 is stable for several weeks at 2-8 ° C, but must be kept at -20 ° C for long-term storage, preferably desiccated. After reconstitution, the preparation is stable for up to one week at 2-8 ° C. For maximum stability, distribute the reconstituted preparation into working aliquots and store them at -20 ° C to -70 ° C. Avoid repeated freeze/thaw cycles.

Purity

Greater than 95.0% as determined by:
(a) RP-HPLC analysis.
(b) Analysis by SDS-PAGE.

Biological Activity

The activity is evaluated on the anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 55,000 Units/mg.