Adiponectin (also called Acrp30, AdipoQ) is a secreted adipocyte-specific protein that circulates in plasma. It is induced during adipocyte differentiation and its secretion is stimulated by insulin. The protein has a carboxyl-terminal globular domain and an amino-terminal collagen domain. The gene is mapped to human chromosome 3q27. Human adiponectin shares approximately 83% amino acid identity with that of the mouse and approximately 90% with that of the rat.
Human adiponectin has been used to study the effect of adiponectin on androstenedione secretion and oxidative stress in a rodent model.
Biochemical / Physiological Actions
Adiponectin (also called Acrp30, AdipoQ) has anti-atherosclerotic and anti-inflammatory properties. It plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and the decreased levels are associated with insulin resistance and hyperinsulinemia. It exhibits antidiabetic action by interacting with the adiponectin receptors, AdipoR1 and AdipoR2. Low levels of adiponectin are also associated with hypertension and dyslipidemia. Mutation in the gene at position 276G is associated with endometrial cancer risk.
Lyophilized from a 0.2 µm filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 µg of bovine serum albumin (BSA) per 1 µg of adiponectin.
Centrifuge the vial before opening. Avoid freeze-thaw cycles. Reconstitute in PBS containing at least 0.1% BSA at a concentration of 0.1-1.0 mg / mL. This stock solution can then be diluted in other aqueous buffers and stored at 4 ° C for 1 week or –20 ° C for future use.
The activity of recombinant human adiponectin is measured by its ability to inhibit the proliferation of the M1 mouse myeloid cell line. The ED50 for effect is typically 1 to 7 µg / ml.